Choose the best membrane for your application.

• PES (polyethersulfone) — PES is the ultimate tissue culture membrane. It's fast and clean. PES is low protein binding, so there is less change of removing critical protein from your media. It is hydrophilic, so no external wedding agents or surfactants are needed, resulting in low extractables. And it is fast, so you spend less time waiting.
• SFCA (surfactant-free cellulose acetate) — SFCA membranes use wetting agents, typically polyglycol ethers, to overcome their natural hydrophobicity. These substances have been shown to be toxic to specific cell lines in vitro^1,2,3. The NALGENE surfactant-free cellulose acetate (SFCA) membrane is the ideal choice if you are growing sensitive cells. SFCA is also low protein binding. If you use CA filtration, you owe it to your cell lines to switch to SFCA. It is only available in NALGENE filter units and bottle top filters.
• CN (cellulose nitrate) — CN filter membranes have been around since the 1940s. NALGENE CN membranes are TRITON X® -free and have excellent wetting properties. CN provides fast flow rates for buffers and other aqueous solutions. CN membranes are ideal for filtering and clarifying solutions when protein binding is not a concern.
• NYL (nylon) — NYL has very low levels of extractables but binds protein. It's inherently hydrophilic so it requires no wetting agents. And Nylon as better alcohol resistance than any other MF75 Series membrane. Its toughness makes Nylon the best membrane for special applications.

Extractables

Protein Binding

References: 1. K.L. Harrison, et al, 1990. Embryotoxicity of micropore filters used in liquid sterilization. Journal of In Vitro Fertilization and Embryo Transfer, 7(6): 347-350. 2. J. R. Wakeland, et al, 1982. Toxicity to organ cultured hearts of media prepared with dispoable filter units. In Vitro, 18(8):715-718. 3. F. Engel, 1982. The effect of membrane filters on cultured cells. Journal of Pharmacy and Pharmacology, 34: 283-286.

Choose the configuration, volume, and membrane diameter you need.

90mm diameter membranes provide a larger surface area for faster flow of difficult-to-filter solutions like high serum media, 100% serum, or fluids with a high particulate load.

Filter Units — Complete ready-to-go sterile systems consist of a filter funnel attached to a filter receiver, packaged with a leak-proof closure for storage of your solution after it is filtered.

Volumes available	Membrane Diameters available
115mL*	50mm
150mL	50mm
250mL	50mm
500mL	75mm, 90mm
1000mL	75mm, 90mm

* 115mL filter units are a unitized design. The funnel is not detachable from the receiver.

Bottle-top Filters — A sterile filter funnel, ready to attach to a separate receiver or glass vacuum bottle. Choose 33mm or 45mm neck to match your glass vacuum bottle. 45mm neck fits NALGENE filter receivers.

Volumes available	Membrane Diameters available	Neck Sizes Available
150mL	50mm	33mm, 45mm
500mL	75mm	33mm
1000mL	90mm	33mm

Filter Receivers — Sterile receivers for use with our bottle-top filters or for separate storage of media, buffer or other fluids. Comes with an attached leak-proof closure that reduces pH shift of media.
Volumes Available:

• 150mL
• 250mL
• 500mL
• 1000mL

NALGENE Certified.

• Every lot of MF75 filter units, bottle tops and receivers is NALGENE Certified. Each case contains documentation that the product is sterile, non-pyrogenic, non-cytotoxic and has passed strict performance integrity tests. We guarantee it!
• Sterile shelf is 5 years.

Easy to use new shape.

• The filter unit receivers have an ergonomic design.
• Tapered sides and "grip dimples" make the filter units and receivers easy to grip and handle.
• The wide base improves stability on the bench top.

Convenient identification.

• Lot number, catalog number, membrane type and pore size and expiration date are printed on the product for easy identification and lot traceability.
• Color-coded filter collars tell you at a glance what membrane type you have.
  
• Blue = Polyethersulfone (PES)
• Yellow = Surfactant-free cellulose acetate (SFCA)
• Green = Cellulose nitrate (CN)
• Red = Nylon (NYL)

Graph: Faster Filtration

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