Standard cellulose acetate (CA) membranes use wetting agents, typically polyglycol ethers, to overcome their natural hydrophobicity. These substances have been shown to be toxic to specific cell lines in vitro (1,2,3). The NALGENE surfactant-free cellulose acetate (SFCA) membrane is the ideal choice if you are growing sensitive cells. SFCA is also low protein binding. If you use CA filtration, you owe it to your cell lines to switch to SFCA. It is only available in NALGENE filter units and bottle top filters.

Fewer Extractables
Low Protein Binding

Capacities: 150ml, 250ml, 500ml and 1000ml.
Pore sizes: 0.2µm and 0.45µm
Membrane diameters: 50mm, 75mm and 90mm.

NALGENE MF75 Filter Units with SFCA Membrane

Capacity Pore Size Membrane Diameter No. Per Case NALGENE Cat. No. 150ml 0.2µm 50mm 12 155-0020 150ml 0.45µm 50mm 12 155-0045 250ml 0.2µm 50mm 12 157-0020 250ml 0.45µm 50mm 12 157-0045 500ml 0.2µm 75mm 12 156-4020 500ml 0.45µm 75mm 12 156-4045 500ml 0.2µm 90mm 12 162-0020 500ml 0.45µm 90mm 12 162-0045 1000ml 0.2µm 75mm 12 158-0020 1000ml 0.45µm 75mm 12 158-0045 1000ml 0.2µm 90mm 12 161-0020 1000ml 0.45µm 90mm 12 161-0045

NALGENE MF75 Bottle Tops with SFCA Membrane

Capacity Pore Size Membrane Diameter Fits Bottle Neck Size No. Per Case NALGENE Cat. No. 150ml 0.2µm 50mm 33mm 12 290-3320 150ml 0.45µm 50mm 33mm 12 290-3345 150ml 0.2µm 50mm 45mm 12 290-4520 150ml 0.45µm 50mm 45mm 12 290-4545 500ml 0.2µm 75mm 33mm 12 291-3320 500ml 0.45µm 75mm 33mm 12 291-3345 500ml 0.2µm 75mm 45mm 12 291-4520 500ml 0.45µm 75mm 45mm 12 291-4545 1000ml 0.2µm 90mm 33mm 12 292-3320 1000ml 0.2µm 90mm 45mm 12 292-4520

References:

1. K.L. Harrison, et al, 1990. Embryotoxicity of micropore filters used in liquid sterilization. Journal of in Vitro Fertilization and Embryo Transfer, 7(6): 347-350.
2. J.R. Wakeland, et al, 1982. Toxicity to organ cultured hearts of media prepared with disposable filter units. In Vitro, 18(8): 715-718.
3. F. Engel, 1982. The effect of membrane filters on cultured cells. Journal of Pharmacy and Pharmacology, 34: 283-286.

Other Membrane Types:

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